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Cell line nameSK-LU-1
Accession numberICLC HTL96023
Brief descriptionSpecies: human, Caucasian; Tissue: lung; Tumor: adenocarcinoma, grade III, poorly differentiated
DescriptionSpecies: human, Caucasian female 60 years old; Tissue: lung; Tumor: adenocarcinoma, grade III, poorly differentiated
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 3-5x104 cells/cm2.37C, 5% CO2.
PropertiesHLA phenotype Aw24,Aw32,B27,Bw41
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, G6PD
Tumorigenicitytumorigenic in immunotolerant rats
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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