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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SK-LU-1 |
| Accession number | ICLC HTL96023 |
| Brief description | Species: human, Caucasian; Tissue: lung; Tumor: adenocarcinoma, grade III, poorly differentiated |
| Description | Species: human, Caucasian female 60 years old; Tissue: lung; Tumor: adenocarcinoma, grade III, poorly differentiated |
| Depositor | Obtained from ECACC. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 3-5x104 cells/cm2.37C, 5% CO2. |
| Properties | HLA phenotype Aw24,Aw32,B27,Bw41 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, G6PD |
| Tumorigenicity | tumorigenic in immunotolerant rats |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |