 |
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
|
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
| Cell line name | GI-CA-N |
| Accession number | ICLC HTL96025 |
| Brief description | Species: human, Caucasian; Tumor: neuroblastoma |
| Description | Species: human, Caucasian male 11 months old; Tumor: neuroblastoma; Derived from: a suprarenal gland pleomorphic anaplastic neuroblastoma |
| Depositor | Dr P. Montaldo, G. Gaslini Institute, Genoa, Italy |
| Reference paper | J Cancer Res Clin Oncol 1988;114:636-640 - DOI: 10.1007/BF00398190 - PMID: 3204111 |
| Morphology and growth | continuous culture, grown as monolayer, morphology neuroepithelial-Schwann like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4x104 cells/cm2. The cells are large and grow very slowly, tend to detach as monolayers after trypsinization and are difficult to disaggregate. 37C, 5% CO2. |
| Properties | N-myc not amplified and 1p not deleted. Used for studies on differentiation; adhesion; neurobiology; growth factors. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with G6PD, PepB |
| Karyology | trisomy, 2p deletion |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Int J Cancer 1990;45:578-579 - DOI: 10.1002/ijc.2910450334
- PMID: 2307545
|