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Cell line name8305C
Accession numberICLC HTL96026
Brief descriptionSpecies: human; Tissue: thyroid; Tumor: carcinoma, undifferentiated
DescriptionSpecies: human female 67 years old; Tissue: thyroid; Tumor: carcinoma, undifferentiated
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology large spindle-shaped cells
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:4 once a week using trypsin/EDTA. Doubling time is about 43 hours. Seed at 2-4 x 104 cells/cm2; cell harvest is about 2 x 106 cells/150 cm2 flask. 37C, 5% CO2.
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, MD
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyBiochem Biophys Res Commun 1994;198:948-954 - PMID: 8117301
Cancer Res 1992;52:1369 - PMID: 1737400
Int J Oncol 1994;4:583-586

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