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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | ME-180 |
| Accession number | ICLC HTL96027 |
| Brief description | Species: human, Caucasian; Tissue: cervix; Tumor: carcinoma, epidermoid |
| Description | Species: human, Caucasian female 66 years old; Tissue: cervix; Tumor: carcinoma, epidermoid; Derived from: metastasis to omentum |
| Depositor | Dr P. Russo, Experimental Oncology Dept, IST, Genoa, Italy. |
| Reference paper | J Natl Cancer Inst 1970;45:107-122 - PMID: 4317734 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:9 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2. |
| Properties | HLA: A1, A11; B5(+/-), B40 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Species validation | Validated by isoenzymes: confirmed as human with G6PD, AST, PepB |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Virology 1985;145:313-318 - DOI: 10.1016/0042-6822(85)90164-3
- PMID: 2992153
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