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Interlab Cell Line Collection (ICLC)
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Cell line nameME-180
Accession numberICLC HTL96027
Brief descriptionSpecies: human, Caucasian; Tissue: cervix; Tumor: carcinoma, epidermoid
DescriptionSpecies: human, Caucasian female 66 years old; Tissue: cervix; Tumor: carcinoma, epidermoid; Derived from: metastasis to omentum
DepositorDr P. Russo, Experimental Oncology Dept, IST, Genoa, Italy.
Reference paperJ Natl Cancer Inst 1970;45:107-122 - PMID: 4317734
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:9 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2.
PropertiesHLA: A1, A11; B5(+/-), B40
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Species validationValidated by isoenzymes: confirmed as human with G6PD, AST, PepB
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyVirology 1985;145:313-318 - DOI: 10.1016/0042-6822(85)90164-3 - PMID: 2992153

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