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Cell line nameNCI-H727
Accession numberICLC HTL97001
Brief descriptionSpecies: human, Caucasian; Tissue: lung; Tumor: carcinoma, non small cell
DescriptionSpecies: human, Caucasian female 65 years old; Tissue: lung; Tumor: carcinoma, non small cell
DepositorObtained from ECACC.
Reference paperJ Clin Invest 1987;79:1629-1634 - PMID: 3034978
Morphology and growthmonoclonal continuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x104 cells/cm2; 37C, 5% CO2.
PropertiesThe best differentiated available bronchial carcinoid cell line. The cells express easily detectable levels of p53 mRNA, and are not able to synthesise gastrin releasing peptide (GRP); neuromedin B peptide synthesis (NMB).
DistributionCell line available for distribution. Any commercial use must be negotiated with the University of Texas
Hazard
Species validationValidated by isoenzymes: confirmes as human with AST, G6PD, NP
Passage number60
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1992;52 (Suppl):2732-2736 Science 1989;246:491-494 - PMID: 2554494

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