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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | NCI-H727 |
| Accession number | ICLC HTL97001 |
| Brief description | Species: human, Caucasian; Tissue: lung; Tumor: carcinoma, non small cell |
| Description | Species: human, Caucasian female 65 years old; Tissue: lung; Tumor: carcinoma, non small cell |
| Depositor | Obtained from ECACC. |
| Reference paper | J Clin Invest 1987;79:1629-1634 - DOI: 10.1172/JCI112999 - PMID: 3034978 |
| Morphology and growth | monoclonal continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x104 cells/cm2; 37C, 5% CO2. |
| Properties | The best differentiated available bronchial carcinoid cell line. The cells express easily detectable levels of p53 mRNA, and are not able to synthesise gastrin releasing peptide (GRP); neuromedin B peptide synthesis (NMB). |
| Distribution | Cell line available for distribution. Any commercial use must be negotiated with the University of Texas |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmes as human with AST, G6PD, NP |
| Passage number | 60 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1992;52 (Suppl):2732-2736 - DOI: 10.1016/0006-291x(75)90293-4
Science 1989;246:491-494 - DOI: 10.1126/science.2554494
- PMID: 2554494
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