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Cell line namePA-1
Accession numberICLC HTL97002
Brief descriptionSpecies: human, Caucasian; Tissue: ovary; Tumor: teratocarcinoma
DescriptionSpecies: human, Caucasian female 12 years old; Tissue: ovary; Tumor: teratocarcinoma; Derived from: ascitic fluid
DepositorDr P. Russo, Experimental Oncology Dept, IST, Genoa, Italy.
Reference paperInt J Cancer 1980;25:19-32 - DOI: 10.1002/ijc.2910250104 - PMID: 6931103
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:10 using trypsin/EDTA; seed at 1-3x104 cells/cm2; 37C, 5% CO2.
PropertiesHLA expression: A28; B12.
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with AST, G6PD
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyIn Vitro 1974;10:382 - DOI: 10.1016/0006-291x(75)90293-4 J Natl Cancer Inst 1974;52:921 - DOI: 10.1093/jnci/52.3.921 - PMID: 4524119

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