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Cell line nameCalu-6
Accession numberICLC HTL97003
Brief descriptionSpecies: human, Caucasian; Tissue: lung; Tumor: carcinoma, anaplastic
DescriptionSpecies: human, Caucasian female 61 years old; Tissue: lung; Tumor: carcinoma, anaplastic; Derived from: Derived from anaplastic carcinoma, probably of lung origin
DepositorDr A. Ardizzoni, Medical Oncology Dept, IST, Genoa, Italy.
Reference paperHuman tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like and round cells
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Split subconfluent cultures 1:5-1:10 using trypsin/EDTA (1-2 min); seed at 3-5 x 104 cells/cm2. 37C, 5% CO2
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST, G6PD
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1988;48:3716-3719 - PMID: 3378214
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

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