 |
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
|
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
| Cell line name | Calu-6 |
| Accession number | ICLC HTL97003 |
| Brief description | Species: human, Caucasian; Tissue: lung; Tumor: carcinoma, anaplastic |
| Description | Species: human, Caucasian female 61 years old; Tissue: lung; Tumor: carcinoma, anaplastic; Derived from: Derived from anaplastic carcinoma, probably of lung origin |
| Depositor | Dr A. Ardizzoni, Medical Oncology Dept, IST, Genoa, Italy. |
| Reference paper | Human tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like and round cells |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Split subconfluent cultures 1:5-1:10 using trypsin/EDTA (1-2 min); seed at 3-5 x 104 cells/cm2. 37C, 5% CO2 |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, G6PD |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1988;48:3716-3719 - PMID: 3378214
J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221 - PMID: 327080 |