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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | OVCAR-3 |
| Accession number | ICLC HTL97004 |
| Brief description | Species: human, Caucasian; Tissue: ovary; Tumor: adenocarcinoma |
| Description | Species: human, Caucasian female 60 years old; Tissue: ovary; Tumor: adenocarcinoma; Derived from: ascitic fluid |
| Depositor | Dr P. Russo, Experimental Oncology Dept, IST, Genoa, Italy |
| Reference paper | Cancer Res 1983;43:5379-5389 - PMID: 6604576 |
| Morphology and growth | monoclonal continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA (10-15 minutes); seed at 1-3x104 cells/cm2; 37C, 5% CO2. |
| Properties | cytotoxicity; antitumour testing; hormone sensibility; drug resistance |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with AST, G6PD |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1984;44:5286-5290 - PMID: 6333272
Science 1984;224:994-996 - DOI: 10.1126/science.6372095 - PMID: 6372095 |