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Cell line nameCOLO 800
Accession numberICLC HTL97005
Brief descriptionSpecies: human; Tissue: subcutaneous nodule; Tumor: melanoma
DescriptionSpecies: human male 14 years old; Tissue: subcutaneous nodule; Tumor: melanoma
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast. Cells display remarkable blebbing
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Genet Cytogenet 1993;68:1002
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