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Cell line nameCA46
Accession numberICLC HTL97007
Brief descriptionSpecies: human; Tissue: ascitic fluid; Tumor: lymphoma, Burkitt
DescriptionSpecies: human; Tissue: ascitic fluid; Tumor: lymphoma, Burkitt
DepositorObtained from ECACC.
Reference paperJ Natl Cancer Inst 1980;64:477-483 - PMID: 6243722
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast-like
Culture conditionsRPMI 1640 + 20% FBS + 2mM L-Glutamine; Maintain cultures between 5 and 9 x 105 cells/ml. 37C, 5% CO2
Propertiesbcl-2 mRNA expression; c-myc mRNA expression; complement receptor; surface IgM (kappa) expression; IgM secretion; EBNA negative
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confimed as human with MD,NP.
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyJ Immunol 1982;129:1336-1342 - PMID: 6286763

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