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Cell line nameCAS-1
Accession numberICLC HTL97009
Brief descriptionSpecies: human; Tumor: glioblastoma
DescriptionSpecies: human male 63 years old; Tumor: glioblastoma
DepositorDr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology glial
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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