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Cell line nameIST-SL2
Accession numberICLC HTL97010
Brief descriptionSpecies: human; Tissue: lung; Tumor: carcinoma, small cell
DescriptionSpecies: human male; Tissue: lung; Tumor: carcinoma, small cell; Derived from: pleural effusion
Depositor Dr S. Ferrini, Immunopharmacology Dept, IST at ABC, Genoa, Italy.
Reference paperUnknown
Morphology and growthcontinuous culture, grown in suspension, morphology round cells, clusters
Culture conditionsRPMI 1640 + 10-20% FBS + 2mM L-Glutamine; maintain cultures between 2 - 4 x 10^5 cells/ml; split cultures 1:2-1:3; 37C, 5% CO2.
Propertiesnegative for HLA antigens
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, NP.
Passage number67
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
CommentsAfter thawing, the cells appear very suffering, and grow slowly (thaw with medium supplemented with FBS 20%)

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