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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | IST-SL1 |
| Accession number | ICLC HTL97012 |
| Brief description | Species: human; Tissue: lung; Tumor: carcinoma, small cell |
| Description | Species: human male; Tissue: lung; Tumor: carcinoma, small cell; Derived from: metastatic lymph node |
| Depositor | Dr S. Ferrini Immunopharmacology Dept, IST at ABC, Genoa, Italy. |
| Reference paper | Br J Cancer 1996;74:788-795 - DOI: 10.1038/bjc.1996.437 - PMID: 8795583 |
| Morphology and growth | continuous culture, grown semiadherent, morphology small cells |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:3 using trypsin/EDTA; seed at 2-4x104 cells/cm2; The cells detach very easily but are difficult to disaggregate; 37C, 5% CO2. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP. |
| Passage number | 51 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |