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Cell line nameIST-SL1
Accession numberICLC HTL97012
Brief descriptionSpecies: human; Tissue: lung; Tumor: carcinoma, small cell
DescriptionSpecies: human male; Tissue: lung; Tumor: carcinoma, small cell; Derived from: metastatic lymph node
DepositorDr S. Ferrini Immunopharmacology Dept, IST at ABC, Genoa, Italy.
Reference paperBr J Cancer 1996;74:788-795 - PMID: 8795583
Morphology and growthcontinuous culture, grown semiadherent, morphology small cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:3 using trypsin/EDTA; seed at 2-4x104 cells/cm2; The cells detach very easily but are difficult to disaggregate; 37C, 5% CO2.
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, NP.
Passage number51
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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