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Cell line nameA-172
Accession numberICLC HTL97013
Brief descriptionSpecies: human; Tumor: glioblastoma
DescriptionSpecies: human male 53 years old; Tumor: glioblastoma
DepositorObtained from ECACC.
Reference paperJ Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
Morphology and growthcontinuous culture, grown as monolayer, morphology glial
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Seed at 2-4x104 cells/cm2; split confluent cultures 1:4-1:8 using trypsin/EDTA. 37C, 5% CO2.
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with G6PD, NP
Passage number318
Tumorigenicitynon tumorigenic in anti thymocyte
Karyologyhypertriploid; n = 80, over 20 marker chromosomes per cell
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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