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Cell line nameTHP-1
Accession numberICLC HTL97014
Brief descriptionSpecies: human; Tissue: peripheral blood; Tumor: leukemia, acute monocytic
DescriptionSpecies: human male 1 years old; Tissue: peripheral blood; Tumor: leukemia, acute monocytic
DepositorDr A. Rubartelli, Clinical Pathology Dept, IST, Genoa, Italy.
Reference paperInt J Cancer 1980;26:171-176 - PMID: 6970727
Morphology and growthcontinuous culture, grown in suspension, morphology large, round, single cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 2-9x105 cells/ml. 37C, 5% CO2
Propertiesdifferentiation; lysozyme synthesis; phagocytosis; Fc receptor; IL-1 production; C3b receptor. HLA: A2, A9; B5; DRw1, DRw2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with G6PD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X,Y; vWA: 16; FGA: 24,25; TH01: 8,9.3; D18S51: 13,14; D21S11: 30,31.2; D8S1179: 10,14
Further bibliographyCancer Res 1982;42:1530 - PMID: 6949641
J Immunol 1983;131:1882 - PMID: 6619543

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