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Cell line nameFTC-133
Accession numberICLC HTL97015
Brief descriptionSpecies: human; Tissue: thyroid; Tumor: carcinoma, follicular
DescriptionSpecies: human male 42 years old; Tissue: thyroid; Tumor: carcinoma, follicular; Derived from: a lymph node metastasis
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology polygonal flat to spindle-like
Culture conditionsDMEM + Ham's F12 (1:1) + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-5x104 cells/cm2. 37C, 5% CO2.
PropertiesThe cell line has been used for studies on the function of human thyroid and development of thyroid cancer; expression of 5-deiodinase type I; the cells retained differentiated thyrocyte function and thyrocyte responsiveness to thyrotropin and local active growth factors; thyroglobulin immunoreactivity could be shown in the cytoplasm and EGF receptor immunoreactivity could be visualised on the cell membranes
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with G6PD, NP.
Passage number12
Karyologycomplex chromosomal changes; p53 mutation
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyFront Horm Res 1989;18:56 - DOI: 10.1016/0006-291x(75)90293-4

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