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Cell line nameMEWO
Accession numberICLC HTL97019
Brief descriptionSpecies: human; Tumor: malignant melanoma
DescriptionSpecies: human; Tumor: malignant melanoma
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2
Propertiesmelanin production; virology; Susceptible to: varicella zoster
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyInfect Immun 1978;19:199-203 - DOI: 10.1128/iai.19.1.199-203.1978 - PMID: 203532
CommentsSusceptible to varicella zoster: optimal growth 32C

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