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Cell line nameSK-N-F1
Accession numberICLC HTL97020
Brief descriptionSpecies: human; Tumor: neuroblastoma
DescriptionSpecies: human male; Tumor: neuroblastoma; Derived from: bone marrow metastasis
DepositorObtained from ECACC.
Reference paperJ Natl Cancer Inst 1984;73(1):51 - PMID: 6610792
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like, clumped cells tend to float
Culture conditionsDMEM + 10% FBS + 1% non-essential amino acids + 4mM L-Glutamine; Split confluent coltures 1:3-1:5 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2.
Propertiestransferrin receptor; choline acetyltransferase synthesis; carcinogenesis studies; cytotoxicity assays; neuroblastoma specific peptide secretion.
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyAnticancer Drugs 1993;4:487 - DOI: 10.1097/00001813-199308000-00010 - PMID: 8104544

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