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Cell line nameKYSE-30
Accession numberICLC HTL97022
Brief descriptionSpecies: human, Asiatic; Tissue: oesophagus; Tumor: carcinoma, squamous cell
DescriptionSpecies: human, Asiatic male 64 year; Tissue: oesophagus; Tumor: carcinoma, squamous cell
DepositorObtained from ECACC.
Reference paperCancer 1992;69:277 - PMID: 1728357
Morphology and growthcontinuous culture, grown as monolayer, morphology polygonal
Culture conditionsRPMI 1640 + Ham's F12 (1:1) + 2% FBS + 2 mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1x104 cells/cm2; 37C, 5% CO2.
PropertiesA p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B have been reported. The cells express a large amount of epidermal growth factor receptors
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with G6PD, MD,NP
Passage number340
Karyologyaneuploid
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyInt J Cancer 1994;58:291 - PMID: 7913084
Int J Cancer 1996;65:372 - PMID: 8575860

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