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Cell line nameCaco-2
Accession numberICLC HTL97023
Brief descriptionSpecies: human, Caucasian; Tissue: colon; Tumor: adenocarcinoma
DescriptionSpecies: human, Caucasian male 72 years old; Tissue: colon; Tumor: adenocarcinoma
DepositorDr A. Corsini, Pharmacological Sciences Institute, Milan, Italy.
Reference paperJ Natl Cancer Inst 1977;58:209-214 - DOI: 10.1093/jnci/58.2.209 - PMID: 833871
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2. After thawing, cells might grow slowly. 37C, 5% CO2
Propertieslipid production; small bowel biology; metabolism; lipoprotein
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Tumorigenicitytumorigenic in nude mice
Karyologymodal number 96
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X,Y; vWA: 16,18; FGA: 19; TH01: 6; D18S51: 12; D21S11: 30; D8S1179: 12
Further bibliographyBiochimie 1986;68:1035-1040 - DOI: 10.1016/s0300-9084(86)80177-8 - PMID: 3096381
Biol Cell 1983;47:323-330 Exp Cell Res 1992;200:97-104 - DOI: 10.1016/s0014-4827(05)80076-x - PMID: 1563496
J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221 - PMID: 327080

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