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Cell line nameVA-ES-BJ
Accession numberICLC HTL97024
Brief descriptionSpecies: human; Tissue: skin; Tumor: sarcoma, epitheloid
DescriptionSpecies: human male; Tissue: skin; Tumor: sarcoma, epitheloid; Derived from: metastasis to bone marrow
DepositorObtained from ECACC.
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like, spindle and giant cells
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:4 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2.
PropertiesGM-CSF production; receptors for GM-CSF, AE, AE3, Fas, EMA, vimentin, P53.
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Passage number28
Tumorigenicitytumorigenic in nude mice
Karyology66-68 aneuploidic, pentasomy #7,multiple trisomy
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyInt J Oncol 1995;7:51-56
CommentsThe viability of the cells can be very low after thawing; thaw with medium supplemented with 20% FBS

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