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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | VA-ES-BJ |
| Accession number | ICLC HTL97024 |
| Brief description | Species: human; Tissue: skin; Tumor: sarcoma, epitheloid |
| Description | Species: human male; Tissue: skin; Tumor: sarcoma, epitheloid; Derived from: metastasis to bone marrow |
| Depositor | Obtained from ECACC. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like, spindle and giant cells |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:3-1:4 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. |
| Properties | GM-CSF production; receptors for GM-CSF, AE, AE3, Fas, EMA, vimentin, P53. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Passage number | 28 |
| Tumorigenicity | tumorigenic in nude mice |
| Karyology | 66-68 aneuploidic, pentasomy #7,multiple trisomy |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Int J Oncol 1995;7:51-56 - DOI: 10.1016/0006-291x(75)90293-4 |
| Comments | The viability of the cells can be very low after thawing; thaw with medium supplemented with 20% FBS |