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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | NT2-D1 |
| Accession number | ICLC HTL97025 |
| Brief description | Species: human, Caucasian; Tissue: testis; Tumor: carcinoma, embryonal pluripotent |
| Description | Species: human, Caucasian male 22 years; Tissue: testis; Tumor: carcinoma, embryonal pluripotent; Derived from: metastasis to lung, original line NTERA-2 |
| Depositor | Dr G. Angelini, Biotechnology Dept, IST at ABC, Genoa, Italy. |
| Reference paper | Unknown |
| Morphology and growth | monoclonal continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | DMEM (4.5 g/L glucose) + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-5x104 cells/cm2; 37C, 5% CO2. After thawing the cells look very suffering and need some days to recover. |
| Properties | The cell line differentiates when exposed to retinoic acid (RA) or hexamethylene bisacetamide (HMBA). |
| Distribution | Cell line available for distribution, as growing culture. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with NP |
| Validated by HLA-DP beta: confirmed as human by PCR | |
| Passage number | 22 |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Dev Biol 1984;103:285-293
Lab Invest 1984;50:147-162 - PMID: 6694356
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