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Cell line nameNT2-D1
Accession numberICLC HTL97025
Brief descriptionSpecies: human, Caucasian; Tissue: testis; Tumor: carcinoma, embryonal pluripotent
DescriptionSpecies: human, Caucasian male 22 years; Tissue: testis; Tumor: carcinoma, embryonal pluripotent; Derived from: metastasis to lung, original line NTERA-2
DepositorDr G. Angelini, Biotechnology Dept, IST at ABC, Genoa, Italy.
Reference paperUnknown
Morphology and growthmonoclonal continuous culture, grown as monolayer, morphology epithelial
Culture conditionsDMEM (4.5 g/L glucose) + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-5x104 cells/cm2; 37C, 5% CO2. After thawing the cells look very suffering and need some days to recover.
PropertiesThe cell line differentiates when exposed to retinoic acid (RA) or hexamethylene bisacetamide (HMBA).
DistributionCell line available for distribution, as growing culture. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with NP
Validated by HLA-DP beta: confirmed as human by PCR
Passage number22
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyDev Biol 1984;103:285-293 Lab Invest 1984;50:147-162 - PMID: 6694356

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