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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | DBTRG-05MG |
| Accession number | ICLC HTL98001 |
| Brief description | Species: human; Tissue: brain; Tumor: glioblastoma multiforme |
| Description | Species: human female; Tissue: brain; Tumor: glioblastoma multiforme |
| Depositor | Obtained from ECACC. |
| Reference paper | In Vitro 1992;28A:609 - DOI: 10.1007/BF02631035 - PMID: 1331021 |
| Morphology and growth | continuous culture, grown as monolayer, morphology glial |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2; 37C, 5% CO2 |
| Properties | HLA class I expressed |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Karyology | 2n=87-91; hypotetraploid |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |