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Cell line nameRO82-W-1
Accession numberICLC HTL98002
Brief descriptionSpecies: human; Tissue: thyroid; Tumor: carcinoma, follicular
DescriptionSpecies: human; Tissue: thyroid; Tumor: carcinoma, follicular
Depositorobtained from ECACC
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + Ham's F12 + MCDB 105 (2:1:1) + 10% FBS + 2 mM L-Glutamine; split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesproduces thyroglobulin; the basal release of immuno-detectable thyroglobulin increased in response to thyroid-stimulating hormone (TSH)
DistributionCell line available for distribution, only as frozen ampoule. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyArch B Cell Pathol 1989;57:167

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