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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | RO82-W-1 |
| Accession number | ICLC HTL98002 |
| Brief description | Species: human; Tissue: thyroid; Tumor: carcinoma, follicular |
| Description | Species: human; Tissue: thyroid; Tumor: carcinoma, follicular |
| Depositor | obtained from ECACC |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + Ham's F12 + MCDB 105 (2:1:1) + 10% FBS + 2 mM L-Glutamine; split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | produces thyroglobulin; the basal release of immuno-detectable thyroglobulin increased in response to thyroid-stimulating hormone (TSH) |
| Distribution | Cell line available for distribution, only as frozen ampoule. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Arch B Cell Pathol 1989;57:167 |