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Cell line nameT47D
Accession numberICLC HTL98003
Brief descriptionSpecies: human; Tissue: breast; Tumor: carcinoma, ductal
DescriptionSpecies: human female 54 years old; Tissue: breast; Tumor: carcinoma, ductal; Derived from: T47, originally derived from pleural effusion
DepositorDr U. Pfeffer, Molecular Biology Dept IST at ABC, Genoa, Italy
Reference paperBiochem Biophys Res Commun 1981;101:1131-1138 - DOI: 10.1016/0006-291x(81)91565-5 - PMID: 6272774
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesreceptors for a variety of steroids and calcitonin
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X; vWA: 14,15; FGA: 23; TH01: 6; D18S51: 17; D21S11: 28,31; D8S1179: 13
Further bibliographyBiochem J 1981;200:315-320 - DOI: 10.1042/bj2000315 - PMID: 6896147

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