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Cell line nameTT
Accession numberICLC HTL98004
Brief descriptionSpecies: human; Tissue: thyroid; Tumor: carcinoma, medullary
DescriptionSpecies: human female 77 years old; Tissue: thyroid; Tumor: carcinoma, medullary
DepositorDr.T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Reference paperRobbins J., Andreoli M., Monaco F. eds., Advances in thyroid neoplasia, Rome: Field Educational Italia; 1981; pp 95-105
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsHam's F12 + 15% FBS + 2mM L-Glutamine; split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 1-3 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesproduces high levels of calcitonin and carcinoembryonic antigen (CEA)
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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