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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | TT |
| Accession number | ICLC HTL98004 |
| Brief description | Species: human; Tissue: thyroid; Tumor: carcinoma, medullary |
| Description | Species: human female 77 years old; Tissue: thyroid; Tumor: carcinoma, medullary |
| Depositor | Dr.T. Florio, Neuroscience Unit, ABC, Genoa, Italy |
| Reference paper | Robbins J., Andreoli M., Monaco F. eds., Advances in thyroid neoplasia, Rome: Field Educational Italia; 1981; pp 95-105 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | Ham's F12 + 15% FBS + 2mM L-Glutamine; split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 1-3 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | produces high levels of calcitonin and carcinoembryonic antigen (CEA) |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |