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Cell line nameGLC-82
Accession numberICLC HTL98006
Brief descriptionSpecies: human, Chinese; Tissue: lung; Tumor: adenocarcinoma
DescriptionSpecies: human, Chinese female 58 years old; Tissue: lung; Tumor: adenocarcinoma
DepositorDr Liu Guizhong, Cancer Institute Panjiayuan, Beijing, China
Reference paperActa Acad Med Kurning 1983;5(1):1-6
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2; DT 48h
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with MD, NP
Karyology63 to 65 chromosomes in 75% of the cells
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyZhonghua Zhong Liu Za Zhi 1985;7:81-2 - PMID: 4006684

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