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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | GLC-82 |
Accession number | ICLC HTL98006 |
Brief description | Species: human, Chinese; Tissue: lung; Tumor: adenocarcinoma |
Description | Species: human, Chinese female 58 years old; Tissue: lung; Tumor: adenocarcinoma |
Depositor | Dr Liu Guizhong, Cancer Institute Panjiayuan, Beijing, China |
Reference paper | Acta Acad Med Kurning 1983;5(1):1-6 |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2; DT 48h |
Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
Karyology | 63 to 65 chromosomes in 75% of the cells |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
Further bibliography | Zhonghua Zhong Liu Za Zhi 1985;7:81-2 - PMID: 4006684
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