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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | BGC-823 |
| Accession number | ICLC HTL98007 |
| Brief description | Species: human, Chinese; Tissue: stomach; Tumor: adenocarcinoma, undifferentiated |
| Description | Species: human, Chinese male 62 years old; Tissue: stomach; Tumor: adenocarcinoma, undifferentiated |
| Depositor | Dr Liu Guizhong, Cancer Institute Panjiayuan, Beijing, China |
| Reference paper | J Beijing Medical College 1986;18(2):136-139 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | CEA positive |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Karyology | 56 to 65 chromosomes in 75% of the cells |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Zhonghua Zhong Liu Za Zhi 1987;9:179-182 - PMID: 3447858
Zhonghua Zhong Liu Za Zhi 1996;18:10-12 - PMID: 8732102 |