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Cell line nameDMS-79
Accession numberICLC HTL98009
Brief descriptionSpecies: human; Tissue: lung, pleural effusion; Tumor: carcinoma, small cell
DescriptionSpecies: human; Tissue: lung, pleural effusion; Tumor: carcinoma, small cell
DepositorObtained from ECACC
Reference paperCancer 1980;45:906-918 - PMID: 6266631
Morphology and growthcontinuous culture, grown in suspension, morphology irregularly shaped aggregates
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; split 1:2-1:4; seed at 1-2 x 10^5 cells/cm2; 37C, 5% CO2
Propertiesthe cell line has been reported to produce ACTH and related peptides, and calcitonin. HLA class I and II expressed
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Karyologyaneuploid: the modal chromosome number tends to decline
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
CommentsThe viability may be low after thawing; thaw the cells with medium supplemented with FBS 20%.

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