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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | DMS-79 |
| Accession number | ICLC HTL98009 |
| Brief description | Species: human; Tissue: lung, pleural effusion; Tumor: carcinoma, small cell |
| Description | Species: human; Tissue: lung, pleural effusion; Tumor: carcinoma, small cell |
| Depositor | Obtained from ECACC |
| Reference paper | Cancer 1980;45:906-918 - DOI: 10.1002/1097-0142(19800301)45:5<906::aid-cncr2820450513>3.0.co;2-h - PMID: 6266631 |
| Morphology and growth | continuous culture, grown in suspension, morphology irregularly shaped aggregates |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; split 1:2-1:4; seed at 1-2 x 10^5 cells/cm2; 37C, 5% CO2 |
| Properties | the cell line has been reported to produce ACTH and related peptides, and calcitonin. HLA class I and II expressed |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Karyology | aneuploid: the modal chromosome number tends to decline |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | The viability may be low after thawing; thaw the cells with medium supplemented with FBS 20%. |