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Cell line nameGI-ME-N
Accession numberICLC HTL98011
Brief descriptionSpecies: human, Caucasian; Tissue: bone marrow; Tumor: metastatic neuroblastoma, stage IV
DescriptionSpecies: human, Caucasian female 2 years old; Tissue: bone marrow; Tumor: metastatic neuroblastoma, stage IV
DepositorDr G.P. Tonini, Population Genetics Dept, IST at ABC, Genoa, Italy
Reference paperPediatr Res 1990;27:1-6 - PMID: 2296463
Morphology and growthcontinuous culture, grown as monolayer, morphology neuroblast-like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesundetectable N-myc expression; the cells do not express HLA Class I
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by isoenzymes: confirmed as human with AST
Validated by HLA-DP beta: confirmed as human by PCR
Tumorigenicityhighly tumorigenic in nude mice
Karyologychromosome marker: 1p del
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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