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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | OC 316 |
| Accession number | ICLC HTL98014 |
| Brief description | Species: human; Tissue: ovary; Tumor: adenocarcinoma, low differentiated; G3 FIGO stage IV (pleural metastasis) |
| Description | Species: human female 60 years old; Tissue: ovary; Tumor: adenocarcinoma, low differentiated; G3 FIGO stage IV (pleural metastasis); Derived from: ascitic fluid |
| Depositor | Dr A. Alama, Pharmacology Dept, IST, Genoa, Italy |
| Reference paper | Gynecol Oncol 1996;62:82-88 - DOI: 10.1006/gyno.1996.0194 - PMID: 8690298 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | RPMI 1640 + 5% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 3-6x10^4 cells/cm2; 37C, 5% CO2 |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Passage number | 43 |
| Tumorigenicity | highly tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |