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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HT-1080 |
| Accession number | ICLC HTL98016 |
| Brief description | Species: human, Caucasian; Tissue: acetabulum; Tumor: fibrosarcoma |
| Description | Species: human, Caucasian male 35 years old; Tissue: acetabulum; Tumor: fibrosarcoma |
| Depositor | Prof. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy |
| Reference paper | Cancer 1974;33:1027-1033 - DOI: 10.1002/1097-0142(197404)33:4<1027::aid-cncr2820330419>3.0.co;2-z - PMID: 4132053 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | Susceptible to: poliovirus 1, vesic. stomatitis (Indiana), feline leukemia, RD114, RNA tumor viruses |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |