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Cell line nameHT-1080
Accession numberICLC HTL98016
Brief descriptionSpecies: human, Caucasian; Tissue: acetabulum; Tumor: fibrosarcoma
DescriptionSpecies: human, Caucasian male 35 years old; Tissue: acetabulum; Tumor: fibrosarcoma
DepositorProf. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy
Reference paperCancer 1974;33:1027-1033 - DOI: 10.1002/1097-0142(197404)33:4<1027::aid-cncr2820330419>3.0.co;2-z - PMID: 4132053
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
PropertiesSusceptible to: poliovirus 1, vesic. stomatitis (Indiana), feline leukemia, RD114, RNA tumor viruses
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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