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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SiHa |
| Accession number | ICLC HTL98017 |
| Brief description | Species: human, Japanese; Tissue: cervix; Tumor: carcinoma, squamous cell |
| Description | Species: human, Japanese female 55 years old; Tissue: cervix; Tumor: carcinoma, squamous cell |
| Depositor | Dr. I. Bongarzone, Istituto Nazionale Tumori, Milan, Italy |
| Reference paper | Proc Soc Exp Biol Med 1970;135:543-545 - DOI: 10.3181/00379727-135-35091a - PMID: 5529598 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; spilt confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Species validation | Validated by isoenzymes: confirmed as human with MD, Pep B |
| Validated by HLA-DP beta: confirmed as human by PCR | |
| Freezing medium | MEM + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Am J Pathol 1985;119:361-366 - PMID: 2990217
Cancer Res 1997;57:2112-2115 - PMID: 9187105 Cancer Res 1997;57:5528-5533 - PMID: 9407963 J Virol 1987;61:962-971 - DOI: 10.1128/JVI.61.4.962-971.1987 - PMID: 3029430 Proc Natl Acad Sci USA 1991;88:5523-5527 - DOI: 10.1073/pnas.88.13.5523 - PMID: 1648218 Virology 1985;145:313-318 - DOI: 10.1016/0042-6822(85)90164-3 - PMID: 2992153 |