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Cell line nameSiHa
Accession numberICLC HTL98017
Brief descriptionSpecies: human, Japanese; Tissue: cervix; Tumor: carcinoma, squamous cell
DescriptionSpecies: human, Japanese female 55 years old; Tissue: cervix; Tumor: carcinoma, squamous cell
DepositorDr. I. Bongarzone, Istituto Nazionale Tumori, Milan, Italy
Reference paperProc Soc Exp Biol Med 1970;135:543-545 - PMID: 5529598
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; spilt confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Species validationValidated by isoenzymes: confirmed as human with MD, Pep B
Validated by HLA-DP beta: confirmed as human by PCR
Freezing mediumMEM + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyAm J Pathol 1985;119:361-366 - PMID: 2990217
Cancer Res 1997;57:2112-2115 - PMID: 9187105
Cancer Res 1997;57:5528-5533 - PMID: 9407963
J Virol 1987;61:962-971 - PMID: 3029430
Proc Natl Acad Sci USA 1991;88:5523-5527 - PMID: 1648218
Virology 1985;145:313-318 - PMID: 2992153

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