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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | CaSki |
| Accession number | ICLC HTL98018 |
| Brief description | Species: human, Caucasian; Tissue: cervix; Tumor: carcinoma, epidermoid, from metastatic site |
| Description | Species: human, Caucasian female 40 years old; Tissue: cervix; Tumor: carcinoma, epidermoid, from metastatic site |
| Depositor | Dr I. Bongarzone, Istituto Nazionale Tumori, Milan, Italy |
| Reference paper | Science 1977;196:1456-1458 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x10^4 cells/cm2; 37C, 5% CO2 |
| Properties | The cells have reported to secrete the beta subunit of human chorionic gonadotropin (beta-hCG) and express tumor-associated antigen |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Passage number | 253 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |