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Cell line nameG-361
Accession numberICLC HTL99001
Brief descriptionSpecies: human; Tissue: skin; Tumor: melanoma
DescriptionSpecies: human male 31 years old; Tissue: skin; Tumor: melanoma
DepositorDr S. Barlati, Biology and Genetics Dept, University of Brescia, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4x10^4 cells/cm2;37C, 5% CO2
PropertiesThe cells produce tissue-type plasminogen activator (tPA)
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyFEBS Lett 1995;363:170-174 - PMID: 7537224
Invasion Metastasis 1995;15:22-23 - PMID: 7672929
Pediatr Res 1978;12:485

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