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Interlab Cell Line Collection (ICLC)
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Cell line nameA-204
Accession numberICLC HTL99002
Brief descriptionSpecies: human; Tissue: muscle; Tumor: rhabdomyosarcoma
DescriptionSpecies: human female 1 year old; Tissue: muscle; Tumor: rhabdomyosarcoma
DepositorProf. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy
Reference paperJ Natl Cancer Inst 1973;51:1417-1423 - DOI: 10.1093/jnci/51.5.1417 - PMID: 4357758
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2 -4x10^4 cells/cm2; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
CommentsThe cells are difficult to detach

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