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Cell line nameMG-63
Accession numberICLC HTL99003
Brief descriptionSpecies: human; Tumor: osteosarcoma
DescriptionSpecies: human male 14 years old; Tumor: osteosarcoma
DepositorProf. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy
Reference paperAntimicrob Agents Chemother 1977;12:11-15 - DOI: 10.1128/AAC.12.1.11 - PMID: 883813
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
Propertiesthe cells are reported to produce high levels of interferon following superinduction with polyinosinic acid-polycytidilic acid, cycloheximide and actinomycin D
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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