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Cell line nameHCT-8
Accession numberICLC HTL99005
Brief descriptionSpecies: human; Tissue: intestine, ileocecal; Tumor: adenocarcinoma
DescriptionSpecies: human male 67 years old; Tissue: intestine, ileocecal; Tumor: adenocarcinoma
DepositorDr A. Sobrero, Udine, Italy
Reference paperJ Natl Cancer Inst 1974;52:1101-1110
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsRPMI 1640 + 10% HS + 2mM L-Glutamine; split confluent cultures 1:5-1:20 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
PropertiesCEA production; the cell line was demonstrated by cytogenetic analysis to be identical to HRT-18 cell line
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyInt J Cancer 1975;16:74-82

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