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Cell line nameZR-75-1
Accession numberICLC HTL99006
Brief descriptionSpecies: human, Caucasian; Tissue: breast; Tumor: carcinoma, ductal
DescriptionSpecies: human, Caucasian female 63 years old; Tissue: breast; Tumor: carcinoma, ductal
DepositorProf. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy
Reference paperCancer Res 1978;38:3352-3364 - PMID: 688225
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
Propertiespositive for estrogen receptor (ER+)
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1978;38:4327-4339 - PMID: 212193
Int J Food Sci Nutr 2000;51:S95-103 - PMID: 11271861

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