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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | ACHN |
| Accession number | ICLC HTL99007 |
| Brief description | Species: human, Caucasian; Tissue: kidney; Tumor: adenocarcinoma |
| Description | Species: human, Caucasian male 22 years old; Tissue: kidney; Tumor: adenocarcinoma; Derived from: pleural effusion |
| Depositor | Prof. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2x104 cells/cm2; 37C, 5% CO2 |
| Properties | antiproliferative studies, using human interferons or interferon inducers |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1987;47:4582-4589 - PMID: 2957045
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