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Cell line nameACHN
Accession numberICLC HTL99007
Brief descriptionSpecies: human, Caucasian; Tissue: kidney; Tumor: adenocarcinoma
DescriptionSpecies: human, Caucasian male 22 years old; Tissue: kidney; Tumor: adenocarcinoma; Derived from: pleural effusion
DepositorProf. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-2x104 cells/cm2; 37C, 5% CO2
Propertiesantiproliferative studies, using human interferons or interferon inducers
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1987;47:4582-4589 - PMID: 2957045

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