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Cell line nameSW1353
Accession numberICLC HTL99008
Brief descriptionSpecies: human, Caucasian; Tissue: bone; Tumor: chondrosarcoma
DescriptionSpecies: human, Caucasian female 72 years old; Tissue: bone; Tumor: chondrosarcoma
DepositorDr T. Florio, Neuroscience Unit, ABC, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology large cells, fibroblast-like
Culture conditionsL-15 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:2-1:3 weekly using trypsin/EDTA; incubate at 37C without CO2 (close the flask caps)
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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