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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | IM-9 |
| Accession number | ICLC HTL99009 |
| Brief description | Species: human; Tissue: bone marrow |
| Description | Species: human female; Tissue: bone marrow; Derived from: a patient with multiple myeloma |
| Depositor | Dr D. Lattuada, Dept of Pharmacology, Chemotherapy and Medical Toxicology, Milan, Italy |
| Reference paper | Ann N Y Acad Sci 1972, 190:221-234 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast-like, single and clumped cells |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 2-9 x 10^5 cells/ml; 37C, 5% CO2 |
| Properties | the cells express surface markers and receptors characteristics of B-lymphoblasts, are capable of synthesizing immunoglobulin and display receptors for human growth hormone, insulin and calcitonin |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Nature 1974, 251:443-444 |