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Cell line nameIM-9
Accession numberICLC HTL99009
Brief descriptionSpecies: human; Tissue: bone marrow
DescriptionSpecies: human female; Tissue: bone marrow; Derived from: a patient with multiple myeloma
DepositorDr D. Lattuada, Dept of Pharmacology, Chemotherapy and Medical Toxicology, Milan, Italy
Reference paperAnn N Y Acad Sci 1972, 190:221-234
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast-like, single and clumped cells
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 2-9 x 10^5 cells/ml; 37C, 5% CO2
Propertiesthe cells express surface markers and receptors characteristics of B-lymphoblasts, are capable of synthesizing immunoglobulin and display receptors for human growth hormone, insulin and calcitonin
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyNature 1974, 251:443-444

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