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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | LNCap.FGC |
| Accession number | ICLC HTL99011 |
| Brief description | Species: human, Caucasian; Tissue: prostate; Tumor: adenocarcinoma |
| Description | Species: human, Caucasian male 50 years old; Tissue: prostate; Tumor: adenocarcinoma; Derived from: metastasis to lymph node |
| Depositor | Dr P. Limonta, Institute of Endocrinology, Milan, Italy |
| Reference paper | Cancer Res 1983;43:1809-1818 - PMID: 6831420 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like, cells growing as aggregates and single cells |
| Culture conditions | RPMI 1640 + 15-20% FBS + 2mM L-Glutamine; split subconfluent cultures 1:2-1:4, do not re-use the same flask; seed at 2-5 x 104 cells/cm2; 37C, 5% CO2 |
| Properties | the cells are reported to produce human prostatic acid phosphatase and prostate specific antigen and to have androgen receptors |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Passage number | 33 |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | 93% FBS + 7% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Models for Prostate Cancer, Alan R. Liss, New York,1981. p.115-132 |
| Comments | the cells are difficult to culture; they grow very slowly, are hard to disaggregate and detach by simply adding PBS; trypsin is used to disaggregate the cells. Thaw the cells with medium supplemented with 20% FBS. More detailed technical instructions will be available on request. |