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Cell line nameLNCap.FGC
Accession numberICLC HTL99011
Brief descriptionSpecies: human, Caucasian; Tissue: prostate; Tumor: adenocarcinoma
DescriptionSpecies: human, Caucasian male 50 years old; Tissue: prostate; Tumor: adenocarcinoma; Derived from: metastasis to lymph node
DepositorDr P. Limonta, Institute of Endocrinology, Milan, Italy
Reference paperCancer Res 1983;43:1809-1818 - PMID: 6831420
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like, cells growing as aggregates and single cells
Culture conditionsRPMI 1640 + 15-20% FBS + 2mM L-Glutamine; split subconfluent cultures 1:2-1:4, do not re-use the same flask; seed at 2-5 x 104 cells/cm2; 37C, 5% CO2
Propertiesthe cells are reported to produce human prostatic acid phosphatase and prostate specific antigen and to have androgen receptors
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Passage number33
Tumorigenicitytumorigenic in nude mice
Freezing medium93% FBS + 7% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyModels for Prostate Cancer, Alan R. Liss, New York,1981. p.115-132
Commentsthe cells are difficult to culture; they grow very slowly, are hard to disaggregate and detach by simply adding PBS; trypsin is used to disaggregate the cells. Thaw the cells with medium supplemented with 20% FBS. More detailed technical instructions will be available on request.

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