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Cell line nameHs913T
Accession numberICLC HTL99012
Brief descriptionSpecies: human, Caucasian; Tumor: fibrosarcoma
DescriptionSpecies: human, Caucasian male 51 years old; Tumor: fibrosarcoma; Derived from: metastasis to lung
DepositorProf. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology fibroblast-like
Culture conditionsDMEM (4.5g/l glucose) + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyAnn Surg Oncol 1996;3:144-149 - PMID: 8646514
J Endocrinol 1999;161:455-464 - PMID: 10333548
Matrix Biol 1995, 14:479-488 - PMID: 7795886

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