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IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
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Cell line nameU-251 MG
Accession numberICLC HTL99014
Brief descriptionSpecies: human, Caucasian; Tumor: glioblastoma-astrocytoma, grade III
DescriptionSpecies: human, Caucasian male 61 years old; Tumor: glioblastoma-astrocytoma, grade III
DepositorObtained from ECACC
Reference paperActa Pathol Microbiol Scand 1968;74:465-486 - DOI: 10.1111/j.1699-0463.1968.tb03502.x - PMID: 4313504
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
DistributionCell line not available for distribution.
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Passage number174
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyHum Hered 1971; 21:238 - DOI: 10.1159/000152408 - PMID: 4332744
CommentsU-251 MG was formerly distributed as U-373 MG until short tandem repeat (STR) genetic profiling confirmed identity with U-251 MG

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