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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | U-251 MG |
| Accession number | ICLC HTL99014 |
| Brief description | Species: human, Caucasian; Tumor: glioblastoma-astrocytoma, grade III |
| Description | Species: human, Caucasian male 61 years old; Tumor: glioblastoma-astrocytoma, grade III |
| Depositor | Obtained from ECACC |
| Reference paper | Acta Pathol Microbiol Scand 1968;74:465-486 - DOI: 10.1111/j.1699-0463.1968.tb03502.x - PMID: 4313504 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Distribution | Cell line not available for distribution. |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Passage number | 174 |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Hum Hered 1971; 21:238 - DOI: 10.1159/000152408
- PMID: 4332744
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| Comments | U-251 MG was formerly distributed as U-373 MG until short tandem repeat (STR) genetic profiling confirmed identity with U-251 MG |