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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SW620 |
| Accession number | ICLC HTL99015 |
| Brief description | Species: human, Caucasian; Tissue: colon; Tumor: adenocarcinoma |
| Description | Species: human, Caucasian male 51 years old; Tissue: colon; Tumor: adenocarcinoma; Derived from: metastasis to lymph node |
| Depositor | Dr U. Pfeffer, Molecular Biology Unit, IST at ABC, Genoa, Italy |
| Reference paper | Cancer Res 1976;36:4562-4569 - PMID: 1000501 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent culture 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Properties | Products: carcinoembryonic antigen (CEA), transforming growth factor alpha, keratin, matrilysin; p53 (G>A mutation in codon 273) |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Tumorigenicity | highly tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221
- PMID: 327080
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