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Cell line nameSW620
Accession numberICLC HTL99015
Brief descriptionSpecies: human, Caucasian; Tissue: colon; Tumor: adenocarcinoma
DescriptionSpecies: human, Caucasian male 51 years old; Tissue: colon; Tumor: adenocarcinoma; Derived from: metastasis to lymph node
DepositorDr U. Pfeffer, Molecular Biology Unit, IST at ABC, Genoa, Italy
Reference paperCancer Res 1976;36:4562-4569 - PMID: 1000501
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; split confluent culture 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
PropertiesProducts: carcinoembryonic antigen (CEA), transforming growth factor alpha, keratin, matrilysin; p53 (G>A mutation in codon 273)
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicityhighly tumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyJ Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221 - PMID: 327080

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