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Cell line nameH-EMC-SS
Accession numberICLC HTL99016
Brief descriptionSpecies: human; Tumor: chondrosarcoma
DescriptionSpecies: human; Tumor: chondrosarcoma
DepositorObtained from ECACC
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like and fibroblast-like, some round cells
Culture conditionsDMEM + Ham's F12 (1:1) + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Passage number28
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyJ Med Sciences 1992;19:1
Commentsthe cells also grow in RPMI 1640

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