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Cell line nameBV-173
Accession numberICLC HTL99018
Brief descriptionSpecies: human; Tissue: peripheral blood; Tumor: leukemia, pre-B cell
DescriptionSpecies: human male 45 years old; Tissue: peripheral blood; Tumor: leukemia, pre-B cell
DepositorDr L. Pegoraro, Cytogenetics and Molecular Biology Dept, Turin, Italy
Reference paperJ Natl Cancer Inst 1983;70:447-453 - PMID: 6572735
Morphology and growthcontinuous culture, grown in suspension, morphology small round cells
Culture conditionsRPMI 1640 + 20% FBS + 2mM L-Glutamine; maintain cultures between 0.5 - 1 x 10^6 cells/ml; 37C, 5% CO2
PropertiesThe cells express bcl2 and c-myc mRNA
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Commentsthe cells grow slowly, are quite difficult to culture and should be frozen at not less than 5 x 10^6 cells/ampoule

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