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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | 380 |
| Accession number | ICLC HTL99019 |
| Brief description | Species: human; Tissue: peripheral blood; Tumor: leukemia, pre-B cell |
| Description | Species: human male 15 years old; Tissue: peripheral blood; Tumor: leukemia, pre-B cell; Derived from: a patient with acute lymphoblastic leukemia (ALL) in relapse |
| Depositor | Dr L. Pegoraro, Cytogenetics and Molecular Biology Dept, University of Turin, Italy |
| Reference paper | Proc Natl Acad Sci USA 1984;81:7166-7170 - DOI: 10.1073/pnas.81.22.7166 - PMID: 6334305 |
| Morphology and growth | continuous culture, grown in suspension, morphology round, small cells |
| Culture conditions | RPMI 1640 + 20% FBS + 2mM L-Glutamine; maintain cultures between 1-2 x 10^6 cells/ml; split 1:2-1:3; 37C, 5% CO2 |
| Properties | EBNA negative |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Karyology | (8;14) and (14;18) translocations |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | the cells are very difficult to culture, grow very slowly and should be frozen at not less than 5 x 10^6 cells/ampoule |