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Cell line name380
Accession numberICLC HTL99019
Brief descriptionSpecies: human; Tissue: peripheral blood; Tumor: leukemia, pre-B cell
DescriptionSpecies: human male 15 years old; Tissue: peripheral blood; Tumor: leukemia, pre-B cell; Derived from: a patient with acute lymphoblastic leukemia (ALL) in relapse
DepositorDr L. Pegoraro, Cytogenetics and Molecular Biology Dept, University of Turin, Italy
Reference paperProc Natl Acad Sci USA 1984;81:7166-7170 - PMID: 6334305
Morphology and growthcontinuous culture, grown in suspension, morphology round, small cells
Culture conditionsRPMI 1640 + 20% FBS + 2mM L-Glutamine; maintain cultures between 1-2 x 10^6 cells/ml; split 1:2-1:3; 37C, 5% CO2
PropertiesEBNA negative
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Karyology(8;14) and (14;18) translocations
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Commentsthe cells are very difficult to culture, grow very slowly and should be frozen at not less than 5 x 10^6 cells/ampoule

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