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Cell line nameSW48
Accession numberICLC HTL99020
Brief descriptionSpecies: human, Caucasian; Tissue: colon; Tumor: adenocarcinoma, grade IV
DescriptionSpecies: human, Caucasian female 82 years old; Tissue: colon; Tumor: adenocarcinoma, grade IV
DepositorDr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Reference paperCancer Res 1976;36:4562-4569 - PMID: 1000501
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsL-15 + 10% FBS + 2mM L-Glutamine; split confluent culture 1:3-1:4 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; incubate at 37C without CO2 (close the flask caps)
PropertiesHLA: A23, A33; B27, B35
DistributionCell line available for distribution. For non-commercial investigative use only
Hazard
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyJ Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Commentsthe cells are difficult to detach: at least 15 min. in trypsin or scraper are required

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